E171 (ATCC® 77310)

Organism: Mus musculus, mouse  /  Clone Type: Library  /  Depositors: JL Rubenstein

Permits and Restrictions

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Designations E171
Species Mus musculus, mouse
Depositors JL Rubenstein
Origin
telencephalon, embryonic day 15
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: E61
Intact vector size: 3.800
Type of vector: phagemid
Vector end: EcoRV
Vector end: NotI
Cloning sites: SacI XhoI XbaI SfiI SacII NotI EcoRI EcoRV HindIII ClaI SalI
Polylinker sites: SacI XhoI XbaI SfiI SacII NotI - stuffer - EcoRI EcoRV
HindIII ClaI SalI
Construction: pBluescript SK-
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
Cross references:
Vector
Tissue: telencephalon, embryonic day 15
DNA: cDNA
Digest: Complete
Insert size range: 0.3 - 10 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 100000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 mL)
Comments
The vector is a derivative of pBluescript SK- modified in the polylinker to minimize complementarity between single-stranded forms of SK- and KS- plasmids.
In this vector, transcription from the T7 promoter gives the sense strand.
Directionally cloned library.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Porteus MH, et al. Isolation and characterization of a library of cDNA clones that are preferentially expressed in the embryonic telencephalon. Brain Res. Mol. Brain Res. 12: 7-22, 1992. PubMed: 1372074

Rubenstein JL, et al. Subtractive hybridization system using single-stranded phagemids with directional inserts. Nucleic Acids Res. 18: 4833-4842, 1990. PubMed: 2168539

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