pURSP1 (ATCC® 77293)

Organism: Schizosaccharomyces pombe Lindner, teleomorph, fission yeast  /  Clone Type: Library  /  Depositors: NC Barbet, AM Carr

Designations pURSP1
Species Schizosaccharomyces pombe Lindner, teleomorph, fission yeast
Depositors NC Barbet, AM Carr
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUR19
Intact vector size: 5.500
Type of vector: plasmid
Vector end: SalI
Vector end: SalI
Cloning sites: EcoRI SacI KpnI SmaI BamHI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC19, pON163
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
marker(s): ura4+
promoter: lac
replicon: ars1
replicon: pMB1
Cross references:
Vector
DNA: genomic
Digest: Partial
Insert size range: 2.5 - 10 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 5000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.4 mL)
Comments
Average insert size: 3.1 kb.
References

Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485

Product Sheet
Product Sheet
Product Sheet