LL16NS03 (ATCC® 57758)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

Designations LL16NS03
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
skin fibroblast, male, HSF7 cell line
Vector
Tissue: skin fibroblast, male, HSF7 cell line
Chromosome: 16
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 20.0
Coverage: Clones for "complete" coverage: 16000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Excellent preparation. Resolution from neighboring peaks optimal. Purity 95%, general background 4%, #15 contamination 1%.
Total human DNA hybridizes to 51% of plaques.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 53.
References

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

Product Sheet
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