LA0XNL01 (ATCC® 57750)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

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Designations LA0XNL01
GenBank Definition

Human zinc finger protein 41 (ZNF41) gene, 3' end.

Human neural adhesion molecule L1 mRNA, partial cds.

GenBank Number

M92443

M55271

Species Homo sapiens, human
Depositors LL Deaven
Origin
multiple X-containing 1635 cell line
Vector
Tissue: multiple X-containing 1635 cell line
Chromosome: X
DNA: genomic
Digest: Partial
Insert size range: 10 - 22 kb
Genome Equivalent: 7.0
Coverage: Clones for "complete" coverage: 14500.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Eighty percent of plaques were detected by 32P-labeled total genomic DNA. Two negative plaques contained E. coli stuffer fragments from the arm preparation; stuffers are considered to be the most likely source of 'negative' plaques.
Depth of sequence representation appears to be excellent. Contains the following 3 sequences from different parts of the X chromosome: p19-2, p58-1 (DXS14, ATCC 57128, 57129), and L1.28 (DXS7, ATCC 57302, 57303).
When the library was screened with a HPRT cDNA probe, of 30600 plaques tested, 7 were positive after 2 cycles, and each showed a restriction map characteristic of HPRT.
References

Djabali M, et al. The gene encoding L1, a neural adhesion molecule of the immunoglobulin family, is located on the X chromosome in mouse and man. Genomics 7: 587-593, 1990. PubMed: 2387585

Dietz-Band JN, et al. Isolation, characterization, and physical localization of 33 human X-chromosome RFLP markers. Cytogenet. Cell Genet. 54: 137-141, 1990. PubMed: 1979940

Lubahn DB, et al. Cloning of human andogen receptor complementary DNA and localization to the X chromosome. Science 240: 327-333, 1988. PubMed: 3353727

Larry L Deaven, personal communication

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