LA01NS01 (ATCC® 57738)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Designations LA01NS01
GenBank Definition

Human cytosolic phospholipase A2 gene, promoter and exon 1.

GenBank Number

U11239

Species Homo sapiens, human
Depositors LL Deaven
Origin
hamster (CHO)/human lymphocyte UV20HL10-12 hybrid
Vector
Tissue: hamster (CHO)/human lymphocyte UV20HL10-12 hybrid
Chromosome: 1
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 31.0
Coverage: Clones for "complete" coverage: 41000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
About 10% hamster impurity by in situ hybridization of sorted chromosomes; about 26% hamster impurity by plaque hybridization.
Of 247 plaques that did not hybridize to either total human or hamster DNA, 113 contained human inserts. Of these, 92 mapped unambiguously to chromosome 1, 12 contained repetitive sequences, and were not mapped.
References

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Dracopoli NC, et al. A genetic linkage map of 27 loci from PND to FY on the short arm of human chromosome 1. Am. J. Hum. Genet. 43: 462-470, 1988. PubMed: 2902785

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