LA0XNS01 (ATCC® 57734)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Permits and Restrictions
Permits and Restrictions

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Designations LA0XNS01
Species Homo sapiens, human
Depositors LL Deaven
Origin
hamster (CHO)/human lymphocyte 81P5d hybrid
Applications
This is preserved in a solution containing 10% glycerol and 2.0 M CsCl.
Vector
Tissue: hamster (CHO)/human lymphocyte 81P5d hybrid
Chromosome: X
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 8.5
Coverage: Clones for "complete" coverage: 25000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
About 5% hamster impurity by in situ hybridization to sorted chromosomes, about 25% hamster impurity by plaque hybridization.
This is preserved in a solution containing 10% glycerol and 2.0 M CsCl. The CsCl should be diluted to less than 40 mM before infection.
References

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Product Sheet
Product Sheet
Product Sheet