LA05NS01 (ATCC® 57720)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Permits and Restrictions
Permits and Restrictions

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Designations LA05NS01
Species Homo sapiens, human
Depositors LL Deaven
Origin
hamster (CHO)/human lymphocyte 640-12 hybrid
Applications
This is preserved in a solution containing 10% glycerol and 0.27 M CsCl.
Vector
Tissue: hamster (CHO)/human lymphocyte 640-12 hybrid
Chromosome: 5
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 6.599999904632568
Coverage: Clones for "complete" coverage: 30000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
This is preserved in a solution containing 10% glycerol and 0.27 M CsCl. The CsCl should be diluted to less than 40 mM before infection.
About 4% hamster impurity by in situ hybridization of sorted chromosomes; about 25% hamster impurity by plaque hybridization.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 43.
References

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Product Sheet
Product Sheet
Product Sheet