LA04NS01 (ATCC® 57719)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Permits and Restrictions

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Designations LA04NS01
Species Homo sapiens, human
Depositors LL Deaven
Origin
hamster (CHO)/human lymphocyte UV20HL21-27 hybrid
Applications
This is preserved in a solution containing 10% glycerol and 2.8 M CsCl.
Vector
Tissue: hamster (CHO)/human lymphocyte UV20HL21-27 hybrid
Chromosome: 4
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 1.600000023841858
Coverage: Clones for "complete" coverage: 31000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
This is preserved in a solution containing 10% glycerol and 2.8 M CsCl. The CsCl should be diluted to less than 40 mM before infection.
About 3% hamster impurity by in situ hybridization of sorted chromosomes; about 11% hamster impurity by plaque hybridization.
About 8% hamster impurity.
The library LA04NS01, ATCC 57719, 1.6 genomic equivalents, is distributed together with LA04NSO2, ATCC 57718, 2.3 genomic equivalents, because there is a possibility that a sequence in one may not be found in the other.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 2.3.
References

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

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