LL18NS01 (ATCC® 57710)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

Permits and Restrictions

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Designations LL18NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
skin fibroblast 761 cell line
Vector
Tissue: skin fibroblast 761 cell line
Chromosome: 18
DNA: genomic
Digest: Complete
Insert size range: 2.0 kb average
Genome Equivalent: 72.0
Coverage: Clones for "complete" coverage: 12000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Good resolution and well-isolated #18 peak. Purity estimated by banding: 97%.
Of 20 probes selected at random, 6 inserts were smaller than 300 bp, 11 contained repetitive sequences, and 3 contained unique sequences. Of the latter 3, all mapped to chromosome 18, and 1 contained 3 HindIII fragments contiguous in the genome.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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