LL11NS01 (ATCC® 57704)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

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Designations LL11NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
hamster (CHO)/human lymphocyte UV20HL4 hybrid
Vector
Tissue: hamster (CHO)/human lymphocyte UV20HL4 hybrid
Chromosome: 11
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 4.900000095367432
Coverage: Clones for "complete" coverage: 22000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
The #11 peak was well-isolated from other human peaks, but neighboring hamster peaks (especially F) are close. This was a noisy preparation.
About 48% of the library is human (and 52% hamster) by plaque hybridization.
This library has been amplified once at the ATCC.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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