LL09NS01 (ATCC® 57703)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

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Designations LL09NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
hamster (CHO)/human lymphocyte UV41HL4 hybrid
Vector
Tissue: hamster (CHO)/human lymphocyte UV41HL4 hybrid
Chromosome: 9
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 13.0
Coverage: Clones for "complete" coverage: 23000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
The #9 peak was very well-isolated from other human peaks but some overlap with neighboring hamster peaks. By fluorescence in situ hybridization with sorted chromosomes, there is 17% hamster DNA.
About 62% of the library is human (and 38% hamster) by plaque hybridization.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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