LL06NS01 (ATCC® 57701)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

Designations LL06NS01
GenBank Definition

H.sapiens SOD-2 gene for manganese superoxide dismutase.

GenBank Number

X65965

Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
hamster (CHO)/human lymphocyte UV20HL15-33 hybrid
Applications
By hybridization analysis, there is 15% hamster DNA in the sorted chromosomes.
Vector
Tissue: hamster (CHO)/human lymphocyte UV20HL15-33 hybrid
Chromosome: 6
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 20.0
Coverage: Clones for "complete" coverage: 28000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
The #6 peak overlaps hamster background; narrow window. By hybridization analysis, there is 15% hamster DNA in the sorted chromosomes.
About 77% of the library is human (and 23% hamster) by plaque hybridization.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 27.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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