LL04NS01 (ATCC® 57700)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

Permits and Restrictions

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Designations LL04NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
hamster (CHO)/human lymphocyte UV20HL21-27 hybrid
Vector
Tissue: hamster (CHO)/human lymphocyte UV20HL21-27 hybrid
Chromosome: 4
DNA: genomic
Digest: Complete
Insert size range: 2.0 kb average
Genome Equivalent: 0.800000011920929
Coverage: Clones for "complete" coverage: 31000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
The #4 peak was well-isolated from hamster background with little debris. According to fluorescence in situ hybridization using genomic hamster DNA with sorted chromosomes, there is 17% hamster DNA.
About 83% of the library is human (and 17% hamster) by plaque hybridization.
Of 2315 randomly selected plaques screened: 224 human single copy greater than 600 bp; 1106-human probably repetitive; 595-inserts absent or less than 600 bp; 62-hamster single copy greater than 600 bp; 328-hamster probably repetitive.
There is approximately 20% hamster impurity.
Of 47 unique sequence clones tested, 47 map to chromosome 4. The average insert size is 2.0 kb.
Of the 224 single copy inserts screened, all detected an appropriate HindIII fragment on chromosome 4; some detected additional fragments not on 4; some also cross-hybridized to hamster sequences.
The library LL04NS01, ATCC 57700, 0.8 genomic equivalents, is distributed together with LL04NS02, ATCC 57745, 10.0 genomic equivalents, because there is a possibility that a sequence in one may not be found in the other.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Gilliam TC, et al. Isolation of polymorphic DNA fragments from human chromosome 4. Nucleic Acids Res. 15: 1445-1458, 1987. PubMed: 2881276

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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