lambdaS2T (ATCC® 37546)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LB Klickstein

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Designations lambdaS2T
GenBank Definition

Human mRNA for complement receptor type 1 (CR1, C3b/C4b receptor, CD35).

GenBank Number

Y00816

Species Homo sapiens, human
Depositors LB Klickstein
Origin
tonsil
Applications
The cDNA was synthesized by oligo(dT) priming and 100 ng of DNA size-selected on agarose gels was used in the construction.
Vector
Tissue: tonsil
DNA: cDNA
Insert size range: 2 - 7 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 400000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen
Comments
EcoRI linkers were added (with internal sites protected). The cDNA was synthesized by oligo(dT) priming and 100 ng of DNA size-selected on agarose gels was used in the construction.
Actin clones comprise 0.3% of all recombinants. Proteins for which cDNAs have been isolated from this library include complement receptors CR1, CR2, and B chain of CR3, and leukocyte common antigen LCA.
References

Wong WW, et al. Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4b. Proc. Natl. Acad. Sci. USA 82: 7711-7715, 1985. PubMed: 2933745

Klickstein LB, et al. Human C3b/C4b receptor (CR1). Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristic of C3/C4 binding proteins. J. Exp. Med. 165: 1095-1112, 1987. PubMed: 2951479

Kishimoto TK, et al. Cloning of the beta subunit of the leukocyte adhesion proteins: Homology to an extracellular matrix receptor defines a novel supergene family. Cell 48: 681-690, 1987. PubMed: 3028646

Benoit E De Crombrugghe, personal communication

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