(ATCC® 37483)

Organism: Bos taurus, cow  /  Clone Type: Library  /  Depositors: R Reeves

Permits and Restrictions

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Species Bos taurus, cow
Depositors R Reeves
Origin
lymphocyte, Con A/PMA-stimulated, 15 hr
Applications
EcoRI linkers were used in the construction.
Vector
Tissue: lymphocyte, Con A/PMA-stimulated, 15 hr
DNA: cDNA
Insert size range: > 0.2 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 200000.0
Biosafety Level 1
Comments
Amplified on E. coli Y1088. Titer on Y1090. Wild-type (non-recombinants) lambdagt11 will amplify more than recombinants. The ratio after first amplification changed from 80% recombinant to 50% recombinant.
EcoRI linkers were used in the construction.
References

Young RA, Davis RW. Yeast RNA polymerase II genes: isolation with antibody probes. Science 222: 778-782, 1983. PubMed: 6356359

Reeves R, et al. Molecular cloning of a functional bovine interleukin 2 cDNA. Proc. Natl. Acad. Sci. USA 83: 3228-3232, 1986. PubMed: 3486415

Product Sheet
Product Sheet
Product Sheet