(ATCC® 37386)

Organism: Escherichia coli (Migula) Castellani and Chalmers  /  Clone Type: Library  /  Depositors: SJ Elledge, GC Walker

Permits and Restrictions

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Species Escherichia coli (Migula) Castellani and Chalmers
Depositors SJ Elledge, GC Walker
Applications
The E. coli host must contain the lambda cI gene for the library to complement a host mutation.
Vector
DNA: genomic
Digest: Partial
Insert size range: 12 - 17 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 400.0
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
Use E. coli LE392 for amplification. The E. coli host must contain the lambda cI gene for the library to complement a host mutation. Plasmid pSE103 (ATCC 37390), which contains cI857, can be transformed into strain to be complemented.
This is the second amplification of this library.
Classification Enterobacteriaceae, Escherichia
References

Elledge SJ, Walker GC. Phasmid vectors for identification of genes by complementation of Escherichia coli mutants. J. Bacteriol. 162: 777-783, 1985. PubMed: 2985547

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