pWR3.26 [molecular clone of rhinovirus 14] purified plasmid DNA (ATCC® VRMC-7)

Organism: Human rhinovirus 14, Human rhinovirus 14  /  Clone Type: Clone  /  Depositors: W Lee

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Designations pWR3.26 [molecular clone of rhinovirus 14] purified plasmid DNA
GenBank Definition

Human rhinovirus type 14 polyprotein gene.

GenBank Number

L05355

Species Human rhinovirus 14, Human rhinovirus 14
Depositors W Lee
Applications
contains sequence
Vector Type of vector E. coli
Name of vector:  pUC-19
Intact vector size (kb):  unknown at this time
Markers (ie. AMPR):  AMPR
Cloning site:  The HRV14 cDNA was transplanted into the vector at the StuI site, then additional modifications were made to the viral sequence by site-directed mutagenesis. (See reference article)
Insert Insert description (full-length, etc.): full-length HRV 14cDNA and T7 promoter
Insert size (kb):  7.212 kb
Full/partial sequence available (ie. GenBank #): L05355 (viral sequence)
Markers (genes and/or restriction sites):
HindIII - 3 sites; Fragment lengths: 5338, 2362, 2147
MluI- 1 site; Fragment length: 9847
Biosafety Level 2
Comments
The construct contains a full-length Human Rhinovirus type 14 (HRV14) genome cloned into the StuI site of the pMJ3 vector.
HRV14 cDNA was transplanted into the vector at the StuI site.
The 5' end of the cDNA is two bases (GG) from the transcription initiation site of the T7 promoter. A unique MluI site (ACGCGT) was added after the polyA tail. Full length transcripts can be produced using T7 RNA polymerase. T7 transcripts are half as infective as genomic RNA from virons.
Classification Picornavirus, Rhinovirus, Animal
References

Lee WM, et al. Role of maturation cleavage in infectivity of picornaviruses: activation of an infectosome. J. Virol. 67: 2110-2122, 1993. PubMed: 8383233