HPV-6b plasmid in E. coli (ATCC® 45150)

Organism: human papilloma virus type 6b  /  Clone Type: Clone  /  Depositors: E de Villiers, H zur Hausen

Designations HPV-6b plasmid in E. coli
GenBank Number

X00203

Species human papilloma virus type 6b
Depositors E de Villiers, H zur Hausen
Origin
condyloma acuminatum
Vector

pUC19 (phagemid)
Size (kb): 2.686
Construction: pUC71K
Marker(s): ampR (50 ug/ml)
Features: Insert detection: lacZ'; Promoter: lac; Replicon: pMB1; MCS:
EcoRI...HindIII
Sequencing primers: M13(-21), M13 reverse

Construct size (kb): 10.6
Insert site: BamHI
Excise insert: BamHI

Insert
Gene product: human papilloma virus type 6b genome
Insert size (kb): 7.9
Insert ends: BamHI
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
Purified plasmid DNA: ATCC 45150
Biosafety Level 2
Comments
Full-length genome of pHPV-6b cloned into BamH1 site of pBR322.
Constructed by linearizing the virus at the unique BamHI site (nt 4722) in the coding region for L2.
The depositor has requested that this deposit be distributed exclusively for scientific and not for commercial purposes.
Restriction digests of the clone give the following sizes (kb): BamHI - 8.0, 2.7; BglI - 7.4, 2.2, 1.0. - ATCC staff
Classification Papovavirus, Papillomavirus, recombinant
References

Schwarz E, et al. DNA sequence and genome organization of genital human papillomavirus type 6b. EMBO J. 2: 2341-2348, 1983. PubMed: 6321162

Harald zur Hausen, personal communication

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