pHPV-2 (6-2) (ATCC® 45022)

Organism: human papilloma virus type 2a  /  Clone Type: Clone  /  Depositors: PM Howley

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Designations pHPV-2 (6-2)
Species human papilloma virus type 2a
Depositors PM Howley
Vector
Construct size (kb): 12.30000019073486
Insert
DNA: genomic
Insert lengths(kb): 8.0
Tissue: DNA from human common hand wart
Gene product: human papilloma virus genome
Insert Size (kb) 8.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--8.0, 4.4; BamHI--12.4.
pHPV-2 (6-2) from common wart. Entire genomic DNA linearized at EcoRI site into EcoRI site of pBR322
Under standard conditions, no homology is detectable between HPV-1a, HPV-1b, HPV-2a, and HPV-4.
Under less stringent conditions, the HPV DNAs and BPV-1 demonstrate about 30% base mismatch.
Circular HPV-2a was linearized with EcoRI before cloning.
References

Heilman CA, et al. Cloning of human papilloma virus genomic DNAs and analysis of homologous polynucleotide sequences. J. Virol. 36: 395-407, 1980. PubMed: 6253665

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