AM6 [EC-AM6, pAM6] purified plasmid DNA (ATCC® 45020D)

Organism: Hepatitis B virus, Hepatitis B virus  /  Clone Type: Clone  /  Depositors: WH Hoyer, Georgetown University Medical Center

Permits and Restrictions

View Permits

Designations AM6 [EC-AM6, pAM6] purified plasmid DNA
GenBank Definition

Cloning vector pBR322, complete sequence

GenBank Number

J01749

Species Hepatitis B virus, Hepatitis B virus
Depositors WH Hoyer, Georgetown University Medical Center
Vector Construct size (kb): 7.5
Marker: ampR
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector ends: BamHI
Host range: Escherichia coli
Vector cross reference: DNA Seq. Acc.: J01749
Insert Genome: hepatitis B virus
Gene name: genome, full-length
Strain: serotype adw
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 3.2
Insert Size (kb) 3.200
Related Products
Source culture: ATCC 45020
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information 200 ng dried purified DNA
Comments
The virus was isolated from plasma (subtype adw) from an HBsAg-positive donor. DNA was extracted from purified Dane particles and cloned into the pBR322 vector.

The complete viral genome was linearized with BamHI and inserted into pBR322.

A BamHI digest cuts the insert into fragments of 1.85 kb and 1.35 kb. The 1.35 kb fragment contains coding sequences for HBsAg and most of region P. The 1.85 kb fragment encodes HBcAg and most of the X gene product.

The 1350 bp BamHI fragment encodes both group and subtype-specific determinants of HBsAg.

The AM6 clone is available as ATCC 39630, ATCC 40101, ATCC 45020, and ATCC 45020D. ATCC 40101 and ATCC 45020D are purified plasmid DNA and ATCC 39630 and ATCC 45020 are the plasmid in E. coli.

Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.85, 1.35; EcoRI--7.5; BglII/PstI--5.2, 1.35, 0.96.
Classification Hepadnaviridae, Orthohepadnavirus
References

Moriarty AM, et al. Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector. Proc. Natl. Acad. Sci. USA 78: 2606-2610, 1981. PubMed: 6264484