pEco63 [clone of hepatitis B] in E. coli (ATCC® 31518)

Organism: Hepatitis B virus, Hepatitis B virus  /  Clone Type: Clone  /  Depositors: Regents of the University of California

Permits and Restrictions

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Designations pEco63 [clone of hepatitis B] in E. coli
Species Hepatitis B virus, Hepatitis B virus
Depositors Regents of the University of California
Applications
Blood borne research
Sexually Transmitted Disease Research
Vector
Construct size (kb): 9.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 3.200000047683716
Insert Size (kb) 3.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped:  Frozen glycerol stock of E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.1, 3.5; HindIII--10.
Full-length clone (insert size 3.2 kb). This material is cited in a U.S. and/or other patent Application and may not be used to infringe the patent claims.
Constructed by inserting a full-length genome of hepatitis B cut with EcoRI into EcoRI site of pBR325
Constructed by inserting a full-length genome of hepatitis B virus cut with EcoRI into the EcoRI site of pBR325.
Classification Hepadnaviridae, Orthohepadnavirus
References

Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336

Kate H Murashige, personal communication

Product Sheet
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