pHP1 plasmid in E. coli GC10 (ATCC® MBA-174)

Organism: Homo sapiens, human  /  Depositors: Roel Nusse

Permits and Restrictions

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Designations pHP1 plasmid in E. coli GC10
Species Homo sapiens, human
Depositors Roel Nusse
Applications
The product of this reaction was then PCR amplified again using another forward primer outside the protein encoding domain and a degenerate set of reverse primers to a highly conserved stretch FHWCC.
Vector
Construct size (kb): 3.95
Insert site: SmaI
Insert
DNA: cDNA
Gene: Wingless-type MMTV integration site family member 3, WNT3
Insert size (kb): 1.0
Putative ID
wingless-related MMTV integration site 3
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information  Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.
Comments
This is a clone of the human WNT3 in the pBluescript M13 vector.

The insert was the result of a PCR amplification of mRNA from fetal tissue using a forward primer derived from the first exon of human WNT3 outside the coding domain and a set of degenerate reverse primers to the reverse complement of the mouse Wnt-3 gene corresponding to the last 4 amino acids and the stop codon. The product of this reaction was then PCR amplified again using another forward primer outside the protein encoding domain and a degenerate set of reverse primers to a highly conserved stretch FHWCC.

Restriction digests of the clone gave the following results (in kb): NotI - 4; NotI/EcoRI - 3.0, 1.0; HindIII/XhoI - 4.0; SmaI - uncut; NotI/HindIII - 3.0, 1.0; HindIII - 4.0. - ATCC staff

Due to the use of a gene internal 3' primer in the PCR reaction, the depositor did not retrieve the complete protein encoding sequence of human WNT3. RefRoelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993.  

References

Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993.

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