pHG30 (ATCC® 99518)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: H Schachter

Permits and Restrictions

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Designations pHG30
GenBank Number

L36537

Species Homo sapiens, human
Depositors H Schachter
Vector
Construct size (kb): 5.960000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: XhoI
Vector end: XhoI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MGAT2
Gene name: mannosyl (alpha-1,6-)-glycoprotein
beta-1,2-N-acetylglucosaminyltransferase
Contains complete coding sequence?: Y
Chromosome: 14; Localization: 14 q21
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 3.0
Cross references: DNA Seq. Acc.: L36537
Nucleotides 1-2964 of the insert correspond to
nucleotides 451-3414 of L36537.
Insert lengths(kb): 3.0
Gene product: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [MGAT2]
Insert Size (kb) 3.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.6, 1.5, 0.86; SalI--3.6, 2.4; XhoI--3.0 (doublet).
The insert contains the following restriction sites (approximate kb from the 5' end): NotI--0.38; KpnI--0.50; SalI--0.70; PstI--0.75, 1.57.
References

Tan J, et al. The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein. Eur. J. Biochem. 231: 317-328, 1995. PubMed: 7635144

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