pHG30 (ATCC® 99518)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: H Schachter

Permits and Restrictions

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Designations pHG30
GenBank Number

L36537

Species Homo sapiens, human
Depositors H Schachter
Vector
Construct size (kb): 5.960000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: XhoI
Vector end: XhoI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MGAT2
Gene name: mannosyl (alpha-1,6-)-glycoprotein
beta-1,2-N-acetylglucosaminyltransferase
Contains complete coding sequence?: Y
Chromosome: 14; Localization: 14 q21
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 3.0
Cross references: DNA Seq. Acc.: L36537
Nucleotides 1-2964 of the insert correspond to
nucleotides 451-3414 of L36537.
Insert lengths(kb): 3.0
Gene product: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [MGAT2]
Insert Size (kb) 3.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.6, 1.5, 0.86; SalI--3.6, 2.4; XhoI--3.0 (doublet).
The insert contains the following restriction sites (approximate kb from the 5' end): NotI--0.38; KpnI--0.50; SalI--0.70; PstI--0.75, 1.57.
References

Tan J, et al. The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein. Eur. J. Biochem. 231: 317-328, 1995. PubMed: 7635144

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