pCWA274 (ATCC® 99479)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pCWA274
GenBank Number

L27425

Species Homo sapiens, human
Vector
Construct size (kb): 10.35000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DNA-PK(CS)
Gene name: DNA-activated serine/threonine protein kinase (catalytic
subunit)
Contains complete coding sequence?: N
Chromosome: 8; Localization: 8 q11
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 7.392
Cross references: DNA Seq. Acc.: L27425
Nucleotides 1-7393 of the insert correspond to
nucleotides 247-7693 of L27425.
Insert lengths(kb): 7.392000198364258
Gene product: DNA-activated serine/threonine protein kinase (catalytic subunit) [DNA-PK(CS)]
Alleles: -, +
Insert Size (kb) 7.392
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.4, 3.0; BamHI--10.4; BglII--9.3, 1.05; HindIII--6.0, 4.4; SmaI--8.0, 2.4.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--3.06, 5.68; BglII--5.15, 6.00; SmaI--5.26; HindIII--6.01.
PvuII polymorphism was detected in the PCR product generated with upstream primer (nt 6229-6252 of L27425) and downstream primer (compliment of nt 7570-7589).
References

Sipley JD, et al. Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8. Proc. Natl. Acad. Sci. USA 92: 7515-7519, 1995. PubMed: 7638222

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