pCWA274 (ATCC® 99479)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pCWA274
GenBank Number

L27425

Species Homo sapiens, human
Vector
Construct size (kb): 10.35000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DNA-PK(CS)
Gene name: DNA-activated serine/threonine protein kinase (catalytic
subunit)
Contains complete coding sequence?: N
Chromosome: 8; Localization: 8 q11
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 7.392
Cross references: DNA Seq. Acc.: L27425
Nucleotides 1-7393 of the insert correspond to
nucleotides 247-7693 of L27425.
Insert lengths(kb): 7.392000198364258
Gene product: DNA-activated serine/threonine protein kinase (catalytic subunit) [DNA-PK(CS)]
Alleles: -, +
Insert Size (kb) 7.392
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.4, 3.0; BamHI--10.4; BglII--9.3, 1.05; HindIII--6.0, 4.4; SmaI--8.0, 2.4.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--3.06, 5.68; BglII--5.15, 6.00; SmaI--5.26; HindIII--6.01.
PvuII polymorphism was detected in the PCR product generated with upstream primer (nt 6229-6252 of L27425) and downstream primer (compliment of nt 7570-7589).
References

Sipley JD, et al. Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8. Proc. Natl. Acad. Sci. USA 92: 7515-7519, 1995. PubMed: 7638222

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