yRM2196 (ATCC® 99423)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: HC Riethman

Permits and Restrictions
Permits and Restrictions

View Permits

Designations yRM2196
GenBank Number

U11838

Species Homo sapiens, human
Depositors HC Riethman
Vector
Construct size (kb): 190.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTYAC1
Intact vector size: 9.000
Type of vector: YAC
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
restriction site: EcoRI
other: telomere, Tetrahymena, <-
marker(s): URA3
centromere: CEN4
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D12S399
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 12
Genome: Homo sapiens
Gene symbol: D12Z5
Gene name: DNA Segment, repetitive (telomere)
Contains complete coding sequence?: U
Chromosome: 12; Localization: 12 q24.33-qter
Tissue: lymphoblastoid CGM-1 cell line
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 180.0
Cross references: DNA Seq. Acc.: U11838
Nucleotides 1-238 of the insert correspond to
nucleotides 1-238 of U11838.
Insert lengths(kb): 180.0
Tissue: lymphoblastoid CGM-1 cell line
Gene product: DNA Segment, repetitive (telomere) [D12Z5]
Insert Size (kb) 180.0
Media AHC- (AHC plus 40 mg/L tryptophan)
Biosafety Level 1
Shipping Information Distributed: frozen
References

Macina RA, et al. Molecular cloning and RARE cleavage mapping of human 2p, 6q, 8q, 12q, and 18q telomeres. Genome Res. 5: 225-232, 1995. PubMed: 8593610

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