CD70 [CD70.1] (ATCC® 95673)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations CD70 [CD70.1]
GenBank Number

L08096

Species Homo sapiens, human
Applications
produces protein CD70 antigen (CD27 ligand)
Vector
Construct size (kb): 5.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pMT2
Intact vector size: 4.200
Type of vector: plasmid
Vector end: BstXI
Vector end: BstXI
Cloning sites: EcoRI
Polylinker sites:
Construction: pBR322; Ad VAI; Ad VAII;
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
other: non-coding DHFR
promoter: adenovirus major late
replicon: pMB1, SV40
terminator: SV4O
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD70
Other gene symbols: CD70.1; CD27LG; CD27L
Gene name: CD70 antigen (CD27 ligand)
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 p13
Tissue: B-lymphocyte JY cell line, EBV transformed
Type of DNA: cDNA
Insert end: Modification: BstXI adapt
Insert end: Modification: BstXI adapt
Insert size (kb): 0.89
Cross references: DNA Seq. Acc.: L08096
Nucleotides 6-888 of the insert correspond to
nucleotides 19-901 of L08096.
Insert lengths(kb): 0.8899999856948853
Tissue: B lymphocyte (EBV transformed), JY cell line
Gene product: CD70 antigen (CD27 ligand) [CD70]
Target Gene: CD70 antigen (CD27 ligand)
Insert Size (kb) 0.890
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): ApaI--5.4; EcoRI--4.4, 0.96; NheI--3.8, 1.6; SmaI--4.6, 0.76; XhoI--5.4.
The insert contains the following restrictions sites (approximate kb from the 5' end): SmaI--0.05, 0.80; NheI--0.11; XhoI--0.29; ApaI--0.37.
References

Bowman MR, et al. The cloning of CD70 and its identification as the ligand for CD27. J. Immunol. 152: 1756-1761, 1994. PubMed: 8120384

Goodwin RG, et al. Molecular and biological characterization of a ligand for CD27 defines a new family of cytokines with homology to tumor necrosis factor. Cell 73: 447-456, 1993. PubMed: 8387892

Product Sheet
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