BL29-112 (ATCC® 95605)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Designations BL29-112
GenBank Number

T27520

Species Homo sapiens, human
Vector
Construct size (kb): 3.349999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.45
Cross references: DNA Seq. Acc.: T27520; EST: EST112; Primer: T7
Insert lengths(kb): 0.449999988079071
Tissue: BL29 cell line Burkitt's lymphoma
Disease: Burkitt's lymphoma
Gene product: DNA Segment, single copy, expressed
Insert Size (kb) 0.450
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--2.4, 0.54, 0.34; PstI--3.3; HindIII--3.05, 0.30; EcoRI--2.9, 0.44; BamHI--3.3.
Product Sheet
Product Sheet
Product Sheet