BL29-60 (ATCC® 95580)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: R Sanders

Permits and Restrictions

View Permits

Designations BL29-60
GenBank Number

T25334

T25335

Species Homo sapiens, human
Depositors R Sanders
Vector
Construct size (kb): 4.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D3S2969E
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 3
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: T25334; EST: EST060; Primer: T7
DNA Seq. Acc.: T25335; EST: EST060R; Primer: T3
Insert lengths(kb): 1.399999976158142
Tissue: BL29 cell line Burkitt's lymphoma
Disease: Burkitt's lymphoma
Gene product: DNA Segment, single copy, expressed [D3S2969E]
Insert Size (kb) 1.400
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.4; BamHI--4.3; PstI--4.3; SmaI--4.3; HindIII--3.5, 0.4, 0.3.
Product Sheet
Product Sheet
Product Sheet