BL29-14 (ATCC® 95541)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations BL29-14
GenBank Number

T46905

Species Homo sapiens, human
Vector
Construct size (kb): 4.401999950408936
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D18S950E
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 18
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.502
Cross references: DNA Seq. Acc.: T46905; EST: EST014; Primer: T7; THC id:
THC112095
Nucleotides 1-1502 of the insert correspond to
nucleotides 1-1502 of T46905.
Insert lengths(kb): 1.501999974250794
Tissue: BL29 cell line Burkitt's lymphoma
Disease: Burkitt's lymphoma
Gene product: DNA Segment, single copy, expressed [D18S950E]
Insert Size (kb) 1.502
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9, 0.6; BamHI--4.3; HindIII--4.3; BglI--3.2, 1.2; PvuII--2.3, 1.3, 0.5, 0.2.
The insert contains the following restriction sites (approximate kb from the T7 end of the insert): NcoI--0.71; EcoRI--0.91, PvuII--1.2, 1.3; PstI--1.3.
Product Sheet
Product Sheet
Product Sheet