pUCSVK661-1 (ATCC® 87722)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JS Butel, JA Lednicky

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Designations pUCSVK661-1
GenBank Number

AF038616

Species Simian virus 40, Simian virus 40
Depositors JS Butel, JA Lednicky
Applications
contains sequence
in another host, produces protein
Vector
Construct size (kb): 7.833000183105469
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: SV40 virus
Gene name: DNA Segment
Contains complete coding sequence?: Y
Strain: rhesus monkey
Tissue: brain, spleen, and lymph nodes
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 5.147
Cross references: DNA Seq. Acc.: AF038616
Nucleotides 1-5147 of the insert correspond to
nucleotides 1-5147 of AF038616.
Insert lengths(kb): 5.146999835968018
Tissue: brain, spleen, and lymph nodes
Insert Size (kb) 5.147
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1, 2.7; BamHI--4.4, 3.4; SalI--7.8.
Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.44; BspHI--1.82, 2.68; EcoRI--1.69; HindIII--0.95, 1.4, 1.62, 3.38, 3.91, 5.08; NcoI--0.04, 0.24, 0.47; NdeI--3.71, 4.73; PstI--1.90, 3.11; PvuII--0.18, 1.62, 3.41.
First protoarchetypal version of SV40 to be cloned and characterized. It has numerous nucleotide differences from SV40 reference strain 776.
This is a viable clone (viral DNA, when excised from the plasmid using EcoRI, will form virus particles if the DNA is transfected into CV-1 cells or other permissive cells).
Full-length genome of SV40 strain K661-1 (SV40-K661-1) was cloned into pUC-19 at the EcoRI site.
Classification Polyomaviridae, Polyomavirus
References

Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685

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