pCMV-Myc-S1P (ATCC® 87718)

Organism: Cricetus cricetus, hamster  /  Clone Type: Clone  /  Depositors: J Sakai, RB Rawson

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Designations pCMV-Myc-S1P
GenBank Number

AF078105

Species Cricetus cricetus, hamster
Depositors J Sakai, RB Rawson
Applications
in another host, produces protein sterol-regulated site-1 protease
Vector
Construct size (kb): 10.19999980926514
Insert
DNA: cDNA

Insert Information:

Insert size (kb): 4.4

Genome: Hamster

Gene symbol: S1P

Gene name: sterol-regulated site-1 protease

Type of DNA: cDNA

Genbank Accession: AF078105

Insert lengths(kb): 4.400000095367432
Gene product: sterol-regulated site-1 protease [S1P]
Target Gene: sterol-regulated site-1 protease
Insert Size (kb) 4.400
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information

 Frozen glycerol stock of Escherichia coli HB101 containing the plasmid.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XbaI -- 5.8, 4.4; EcoRI--9.7, 0.5; HindIII--6.0, 2.4, 1.8.
Insert encodes an epitope-tagged version of the protein including aa 1-23, three novel amino acids (GGR), three tandem copies of the 9E10 human c-Myc epitop tag, followed by aa 24 - 1052 of the protein.
References

Sakai J, et al. Molecular identification of sterol-regulated luminal protease that cleaves SREBPs and controls lipid composition of animal cells. Mol. Cell 2: 505-514, 1998. PubMed: 9809072

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