pMR1593 (ATCC® 87710)

Applications: cloning vector  /  Depositors: F Spencer

Permits and Restrictions

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Designations pMR1593
Depositors F Spencer
Biosafety Level 1
Vector Information
Size (kb): 7.8000001907348630
Vector: pMR1593 (phage, lambda - replacement)
Marker(s):ampR
Construct size (kb): 7.800000190734863
Features: marker(s): ampR, URA3
replicon: ori
MCS: SalI [delta15]
restriction site: BglII, EcoRI, PstI, NcoI, SalI, HindIII
coding sequence: karldelta15
Applications
cloning vector
Comments
Supports introduction of kar1delta15 mutation into ura3 yeast strains by two step gene replacement. The kar1delta15 mutation supports transfer of YAC clone between yeast strains by Kar1-mating (REF).
To integrate pMR1593, linearize within the kar1 gene at the unique BglII site. Cells that have lost the URA3 marker through mitotic excision can be selected on 5-fluororotic acid.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vallen EA, et al. Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion. J. Cell Biol. 117: 1277-1287, 1992. PubMed: 1607389

Spencer F, et al. Yeast kar1 mutants provide an effective method for YAC transfer to new hosts. Genomics 22: 118-126, 1994. PubMed: 7959757

Sano Y, et al. Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome. J. Gen. Virol. 79: 3111-3118, 1998. PubMed: 9880029

Hugerat Y, et al. A versatile method for efficient YAC transfer between any two strains. Genomics 22: 108-117, 1994. PubMed: 7959756

Green EDYeast artificial chromosomeIn: Green EDGenome analysis: a laboratory manual3in press, 1999

Spencer F, Simchen GTransfer of YAC clones to new hosts by karyogamy deficient mating.In: Spencer F, Simchen GCurrent Protocols in Human GeneticsNew York, N.Y.John Wiley and Sonsin press, 1995

Shipped freeze-dried
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