Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 7.8000001907348630
Vector: pMR1593 (phage, lambda - replacement)
Construct size (kb): 7.800000190734863
Features: marker(s): ampR, URA3
MCS: SalI [delta15]
restriction site: BglII, EcoRI, PstI, NcoI, SalI, HindIII
coding sequence: karldelta15
Supports introduction of kar1delta15 mutation into ura3 yeast strains by two step gene replacement. The kar1delta15 mutation supports transfer of YAC clone between yeast strains by Kar1-mating (REF).
To integrate pMR1593, linearize within the kar1 gene at the unique BglII site. Cells that have lost the URA3 marker through mitotic excision can be selected on 5-fluororotic acid.
Vallen EA, et al. Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion. J. Cell Biol. 117: 1277-1287, 1992. PubMed: 1607389
Spencer F, et al. Yeast kar1 mutants provide an effective method for YAC transfer to new hosts. Genomics 22: 118-126, 1994. PubMed: 7959757
Sano Y, et al. Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome. J. Gen. Virol. 79: 3111-3118, 1998. PubMed: 9880029
Hugerat Y, et al. A versatile method for efficient YAC transfer between any two strains. Genomics 22: 108-117, 1994. PubMed: 7959756
Green EDYeast artificial chromosomeIn: Green EDGenome analysis: a laboratory manual3in press, 1999
Spencer F, Simchen GTransfer of YAC clones to new hosts by karyogamy deficient mating.In: Spencer F, Simchen GCurrent Protocols in Human GeneticsNew York, N.Y.John Wiley and Sonsin press, 1995