Size (kb): 7.8000001907348630
Vector: pMR1593 (phage, lambda - replacement)
Construct size (kb): 7.800000190734863
Features: marker(s): ampR, URA3
MCS: SalI [delta15]
restriction site: BglII, EcoRI, PstI, NcoI, SalI, HindIII
coding sequence: karldelta15
Supports introduction of kar1delta15 mutation into ura3 yeast strains by two step gene replacement. The kar1delta15 mutation supports transfer of YAC clone between yeast strains by Kar1-mating (REF).
To integrate pMR1593, linearize within the kar1 gene at the unique BglII site. Cells that have lost the URA3 marker through mitotic excision can be selected on 5-fluororotic acid.
Vallen EA, et al. Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion. J. Cell Biol. 117: 1277-1287, 1992. PubMed: 1607389
Spencer F, et al. Yeast kar1 mutants provide an effective method for YAC transfer to new hosts. Genomics 22: 118-126, 1994. PubMed: 7959757
Sano Y, et al. Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome. J. Gen. Virol. 79: 3111-3118, 1998. PubMed: 9880029
Hugerat Y, et al. A versatile method for efficient YAC transfer between any two strains. Genomics 22: 108-117, 1994. PubMed: 7959756
Green EDYeast artificial chromosomeIn: Green EDGenome analysis: a laboratory manual3in press, 1999
Spencer F, Simchen GTransfer of YAC clones to new hosts by karyogamy deficient mating.In: Spencer F, Simchen GCurrent Protocols in Human GeneticsNew York, N.Y.John Wiley and Sonsin press, 1995