YEpFAT2 (ATCC® 87640)

Applications: M9 + leucine + tryptophan culture media recommended for DNA purification.  /  Depositors: K Runge

Permits and Restrictions

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Designations YEpFAT2
Depositors K Runge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli MC1066
Vector Information
Size (kb): 7.3769998550415040
Intact vector size: 7.377
Type of vector: plasmid
Cloning sites: NotI SacI SmaI BamHI XbaI
Polylinker sites: NotI EcoRI SacI AvaI KpnI SmaI BamHI XbaI
Other unique sites: HindIII
Host range: Saccharomyces cerevisiae; Escherichia coli;
Schizosaccharomyces pombe
Features (with orientation and position when available):
restriction site: HindIII, 1-1
promoter for in vitro transcription: T3, <-, 11-30
promoter: lac, <-, 100-128
replicon: pMB1, 454-454
marker(s): ampR, <-, 1212-2072
promoter for in vitro transcription: T7, ->, 3132-3151
MCS: NotI...XbaI, ->, 3180-3214
replicon: 2 micron/LEU2-d, 3208-5903
marker(s): LEU2-d, ->, 4570-5664
marker(s): URA4 from S. pombe, <-, 6055-6849
Vector: YEpFAT2 (plasmid)
Promoters: Promoter for in vitro transcription T3
Construct size (kb): 7.376999855041504
Features: marker(s): LEU2-d
marker(s): URA4 from S. pombe
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: NotI...XbaI
restriction site: HindIII
M9 + leucine + tryptophan culture media recommended for DNA purification.
Restriction digests of the clone give the following sizes (kb): BamHI--7.2; SmaI--7.2; EcoRI--5.4, 2.03.
M9 + leucine + tryptophan culture media recommended for DNA purification.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

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