pSON8-JUN (ATCC® 87567)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pSON8-JUN
GenBank Number

J04111

Species Homo sapiens, human
Depositors SJ Elledge
Applications
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm.
Positive control for SOS Recruitment System.
Vector
Construct size (kb): 9.694000244140625
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSON8
Intact vector size: 9.469
Type of vector: plasmid
Vector end: NcoI
Vector end: SalI
Cloning sites: NcoI SmaI HpaI SalI EagI
Polylinker sites: NcoI SmaI HpaI SalI EagI HindIII MunI EcoRI XhoI
Other unique sites: ApaI SpeI NheI BglII KpnI BamHI PvuI XbaI AhaII
Construction: pUC
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->
promoter: ADH, ->
MCS: NcoI...EagI, ->
coding sequence: SOS (human Son of sevenless), ->
terminator: ADH, ->
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron ori
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: JUN
Gene name: c-jun oncogene
Contains complete coding sequence?: N
Chromosome: 1; Localization: 1p32-31
Type of DNA: cDNA
Insert 5' end: NcoI
Insert 3' end: SalI
Insert size (kb): 0.257
Cross references: DNA Seq. Acc.: J04111
Insert lengths(kb): 0.2569999992847443
Gene product: c-jun oncogene [JUN]
Insert Size (kb) 0.257
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): SalI--9.7; HindIII--6.0, 3.7; BamHI--9.7; NcoI/SalI--9.5, 0.25.
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein.
Positive control for SOS Recruitment System.
The insert contains the leucine zipper motif.
References

Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808

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