pSON8-JUN (ATCC® 87567)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pSON8-JUN
GenBank Number

J04111

Species Homo sapiens, human
Depositors SJ Elledge
Applications
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm.
Positive control for SOS Recruitment System.
Vector
Construct size (kb): 9.694000244140625
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSON8
Intact vector size: 9.469
Type of vector: plasmid
Vector end: NcoI
Vector end: SalI
Cloning sites: NcoI SmaI HpaI SalI EagI
Polylinker sites: NcoI SmaI HpaI SalI EagI HindIII MunI EcoRI XhoI
Other unique sites: ApaI SpeI NheI BglII KpnI BamHI PvuI XbaI AhaII
Construction: pUC
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->
promoter: ADH, ->
MCS: NcoI...EagI, ->
coding sequence: SOS (human Son of sevenless), ->
terminator: ADH, ->
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron ori
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: JUN
Gene name: c-jun oncogene
Contains complete coding sequence?: N
Chromosome: 1; Localization: 1p32-31
Type of DNA: cDNA
Insert 5' end: NcoI
Insert 3' end: SalI
Insert size (kb): 0.257
Cross references: DNA Seq. Acc.: J04111
Insert lengths(kb): 0.2569999992847443
Gene product: c-jun oncogene [JUN]
Insert Size (kb) 0.257
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): SalI--9.7; HindIII--6.0, 3.7; BamHI--9.7; NcoI/SalI--9.5, 0.25.
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein.
Positive control for SOS Recruitment System.
The insert contains the leucine zipper motif.
References

Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808

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