Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 5.744
Vector: pJM6 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pJM4, pBluescriptIIKS+
Construct size (kb): 5.74399995803833
Features: marker(s): URA3
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--2.9 doublet; PstI/XhoI--2.9 doublet; XbaI/XhoI--2.9 doublet; HindIII--6.0; XhoI--6.0.
The insert contains the following restriction sites (approximate kb from the 5' end): HpaII--0.76, 1.08; BsmI--0.97; HindIII--1.77; AgeI--1.94.
Yang VW, et al. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl. Environ. Microbiol. 60: 4245-4254, 1994. PubMed: 7811063