pJM6 phagemid in E. coli (ATCC® 87492)

Applications: shuttle vector  /  Depositors: TW Jeffries

Permits and Restrictions

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Designations pJM6 phagemid in E. coli
Depositors TW Jeffries
Biosafety Level 1
Vector Information
Size (kb): 5.744
Vector: pJM6 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pJM4, pBluescriptIIKS+
Marker(s):URA3,ampR
Construct size (kb): 5.74399995803833
Features: marker(s): URA3
marker(s): ampR
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS2
replicon: f1
replicon: pMB1
MCS: SacI/.../EcoRI
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--2.9 doublet; PstI/XhoI--2.9 doublet; XbaI/XhoI--2.9 doublet; HindIII--6.0; XhoI--6.0.
The insert contains the following restriction sites (approximate kb from the 5' end): HpaII--0.76, 1.08; BsmI--0.97; HindIII--1.77; AgeI--1.94.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yang VW, et al. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl. Environ. Microbiol. 60: 4245-4254, 1994. PubMed: 7811063

Shipped freeze-dried
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