pJM6 phagemid in E. coli (ATCC® 87492)

Applications: shuttle vector  /  Depositors: TW Jeffries

Permits and Restrictions

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Designations pJM6 phagemid in E. coli
Depositors TW Jeffries
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.744
Vector: pJM6 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pJM4, pBluescriptIIKS+
Marker(s):URA3,ampR
Construct size (kb): 5.74399995803833
Features: marker(s): URA3
marker(s): ampR
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS2
replicon: f1
replicon: pMB1
MCS: SacI/.../EcoRI
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--2.9 doublet; PstI/XhoI--2.9 doublet; XbaI/XhoI--2.9 doublet; HindIII--6.0; XhoI--6.0.
The insert contains the following restriction sites (approximate kb from the 5' end): HpaII--0.76, 1.08; BsmI--0.97; HindIII--1.77; AgeI--1.94.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yang VW, et al. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl. Environ. Microbiol. 60: 4245-4254, 1994. PubMed: 7811063

Shipped freeze-dried