lambdaTRP (ATCC® 87269)

Applications: expression vectorlambda vector allowing conversion to plasmidvector for constructing cDNA libraries  /  Depositors: SJ Elledge

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Designations lambdaTRP
Depositors SJ Elledge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 42.40
Vector: lambdaTRP (phage, lambda - replacement)
Promoters: Promoter for expression GAL1
Construction: lambdaYES-R, pTRP
Marker(s):TRP1,ampR
Construct size (kb): 42.40
Features: marker(s): TRP1
marker(s): ampR
other: left arm (19.6 kb)
other: lox
other: right arm (15.2 kb, with cI857)
promoter for expression: GAL1
replicon: 2 micron
replicon: oriT
replicon: pMB1
MCS: EcoRI...SacI
transcription terminator: CYC1
Applications
expression vector
lambda vector allowing conversion to plasmid
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--30.0, 18.0; SalI--20.0, 14.0, 13.0.
Transcription initiated from the GAL1 promoter in yeast hosts can be terminated at the CYC1 terminators. The GAL1 promoter lacks a translational start signal. There is also some expression on glucose.
Vector for the construction of cDNA libraries permitting regulated expression in yeast and simple conversion to plasmid via the cre-lox system.
The plasmid derivative (pTRP, 8.0 kb) is a YE-type shuttle expression vector.
Growth Conditions
Temperature: 37.0°C
References

Manoharan HT, et al. High-level production and purification of biologically active proteins from bacterial and mammanlian cells using the tandem pGFLEX expression system. Gene 193: 229-237, 1997. PubMed: 9256081

Stephen J Elledge, personal communication

Shipped freeze-dried
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