Size (kb): 42.40
Vector: lambdaTRP (phage, lambda - replacement)
Promoters: Promoter for expression GAL1
Construction: lambdaYES-R, pTRP
Construct size (kb): 42.40
Features: marker(s): TRP1
other: left arm (19.6 kb)
other: right arm (15.2 kb, with cI857)
promoter for expression: GAL1
replicon: 2 micron
transcription terminator: CYC1
lambda vector allowing conversion to plasmid
vector for constructing cDNA libraries
Restriction digests of the clone give the following sizes (kb): XhoI--30.0, 18.0; SalI--20.0, 14.0, 13.0.
Transcription initiated from the GAL1 promoter in yeast hosts can be terminated at the CYC1 terminators. The GAL1 promoter lacks a translational start signal. There is also some expression on glucose.
Vector for the construction of cDNA libraries permitting regulated expression in yeast and simple conversion to plasmid via the cre-lox system.
The plasmid derivative (pTRP, 8.0 kb) is a YE-type shuttle expression vector.
Manoharan HT, et al. High-level production and purification of biologically active proteins from bacterial and mammanlian cells using the tandem pGFLEX expression system. Gene 193: 229-237, 1997. PubMed: 9256081
Stephen J Elledge, personal communication