pPD101 (ATCC® 87223)

Applications: expression vectormobilization helper plasmid  /  Depositors: E Bremer

Permits and Restrictions

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Designations pPD101
Depositors E Bremer
Biosafety Level 1
Vector Information
Size (kb): 3.5199999809265140
Vector: pPD101 (plasmid)
Promoters: Promoter T7 gene 10
Construction: pHSG576, pT7-6, pUC-BM20
Marker(s):cmlR
Construct size (kb): 3.519999980926514
Features: marker(s): cmlR
promoter: T7 gene 10
replicon: pSC101
MCS: HindIII...EcoRI
Applications
expression vector
mobilization helper plasmid
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; NcoI--2.7, 0.7.
pPD100 (ATCC 87222) and pPD101 (ATCC 87223) require that the insert provide a translational initiation signal for expression. pPD100 differs from pPD101 only in the orientation of the MCS.
Deposited for research purposes only. Before any commercial use, the depositor must be contacted.
A low copy number plasmid using the T7 gene 10 promoter for selective, overexpression of cloned genes in E. coli.
Media Medium 2272: LB Medium (Miller) with chloramphenicol 25 mcg/ml
Growth Conditions
Temperature: 37.0°C
References

Dersch P, et al. Low-copy-number T7 vectors for selective gene expression and efficient protein overproduction in Escherichia coli. FEMS Microbiol. Lett. 123: 19-26, 1994. PubMed: 7988888

Erhard Bremer, personal communication

Shipped freeze-dried
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