pPD101 (ATCC® 87223)

Applications: expression vectormobilization helper plasmid  /  Depositors: E Bremer

Permits and Restrictions

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Designations pPD101
Depositors E Bremer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.5199999809265140
Vector: pPD101 (plasmid)
Promoters: Promoter T7 gene 10
Construction: pHSG576, pT7-6, pUC-BM20
Marker(s):cmlR
Construct size (kb): 3.519999980926514
Features: marker(s): cmlR
promoter: T7 gene 10
replicon: pSC101
MCS: HindIII...EcoRI
Applications
expression vector
mobilization helper plasmid
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; NcoI--2.7, 0.7.
pPD100 (ATCC 87222) and pPD101 (ATCC 87223) require that the insert provide a translational initiation signal for expression. pPD100 differs from pPD101 only in the orientation of the MCS.
Deposited for research purposes only. Before any commercial use, the depositor must be contacted.
A low copy number plasmid using the T7 gene 10 promoter for selective, overexpression of cloned genes in E. coli.
Media ATCC® Medium 2272: LB Medium (Miller) with chloramphenicol 25 mcg/ml
Growth Conditions
Temperature: 37.0°C
References

Dersch P, et al. Low-copy-number T7 vectors for selective gene expression and efficient protein overproduction in Escherichia coli. FEMS Microbiol. Lett. 123: 19-26, 1994. PubMed: 7988888

Erhard Bremer, personal communication

Shipped freeze-dried