Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 6.5999999046325680
Vector: pIIIEx313 tRNA (plasmid)
Promoters: Promoter for in vitro transcription T7
Construct size (kb): 6.599999904632568
Features: insert detection: lacZ'
promoter: SUP4 tRNA intragenic promoter
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
YC-type (centromeric) shuttle vector
vector permitting RNA synthesis in vitro
vector permitting expression of small structured RNAs
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
vector with low copy number
Restriction digests of the clone give the following sizes (kb): BamHI 6.6; SalI--6.6; EcoRI--6.6.
Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences.
One of a series of shuttle vectors (ATCC 87167
- 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.
Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS313 (ATCC 77142)
Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876