YCpIF6 (ATCC® 87083)

Applications: YX-type (expression) shuttle vector  /  Depositors: RW Davis, PK Foreman

Permits and Restrictions

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Designations YCpIF6
Depositors RW Davis, PK Foreman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.1999998092651370
Vector: YCpIF6 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pUN55
Marker(s):URA3,ampR
Construct size (kb): 7.199999809265137
Features: marker(s): URA3
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SalI...SacI
centromere: CEN4
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--7.2; BamHI--7.2; EcoRI--7.2.
Care should be taken when cloning into this vector because several stop codons occur in this reading frame.
One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.
Vectors YCpIF4, YCpIF5 and YCpIF6 (ATCC 87081-87083) differ only in the reading frame of the multiple cloning site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907

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