YCpIF6 (ATCC® 87083)

Applications: YX-type (expression) shuttle vector  /  Depositors: RW Davis, PK Foreman

Permits and Restrictions

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Designations YCpIF6
Depositors RW Davis, PK Foreman
Biosafety Level 1
Vector Information
Size (kb): 7.1999998092651370
Vector: YCpIF6 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pUN55
Marker(s):URA3,ampR
Construct size (kb): 7.199999809265137
Features: marker(s): URA3
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SalI...SacI
centromere: CEN4
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--7.2; BamHI--7.2; EcoRI--7.2.
Care should be taken when cloning into this vector because several stop codons occur in this reading frame.
One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.
Vectors YCpIF4, YCpIF5 and YCpIF6 (ATCC 87081-87083) differ only in the reading frame of the multiple cloning site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907

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