pTC181 (ATCC® 87067)

Applications: vector containing primer sites useful for sequencingvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: A Talavera

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Designations pTC181
Depositors A Talavera
Biosafety Level 1
Vector Information
Size (kb): 2.7000000476837160
Vector: pTC181 (plasmid)
Construction: pUC18
Marker(s):ampR
Construct size (kb): 2.700000047683716
Features: insert detection: lacZ'
marker(s): ampR
replicon: pMB1
MCS: HindIII...BamHI
Applications
vector containing primer sites useful for sequencing
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.15; NcoI--2.7; PvuII--2.3, 0.40.
The plasmid contains the following restriction sites (approximate kb from nt 1): BglI--0.24, 1.85; NcoI--0.45; PvuI--0.28, 2.10; PvuII--0.31, 0.67.
Expression vector designed for converting restriction sites at the end of inserts. Also permits insert detection by lacZalpha complementation and sequencing from M13 universal and reverse primers.
Constructed from pUC18 by modification of the multiple cloning site to include some unique cloning sites surrounded by some direct repeat sites.
Pairs of identical restriction sites are in the same reading frame to maintain lacZ' insert detection regardless of the enzyme used.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

DeLaFuente A, et al. Restriction end-converting vectors with tandem repeated multiple cloning sites. Gene 139: 83-86, 1994. PubMed: 8112593

Shipped freeze-dried
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