lambdahSSTR2-17 (ATCC® 79048)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: GI Bell

Permits and Restrictions

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Designations lambdahSSTR2-17
GenBank Number

M96738

Species Homo sapiens, human
Depositors GI Bell
Vector
Construct size (kb): 44.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaFIX II
Intact vector size: 41.900
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: NotI SalI XhoI EcoRI
Polylinker sites: XbaI SacI NotI SacI SalI XhoI EcoRI XbaI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: T3, T7
replicon: lambda
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SSTR3
Gene name: somatostatin receptor 3
Contains complete coding sequence?: Y
Chromosome: 22; Localization: 22 q13.1
Tissue: placenta, male Caucasian
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 15.0
Cross references: DNA Seq. Acc.: M96738
Insert lengths(kb): 15.0
Tissue: placenta, male Caucasian
Gene product: somatostatin receptor 3 [SSTR3]
Insert Size (kb) 15.0
Biosafety Level 1
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
SacI--18.5, 8.6, 4.4, 2.5, 2.3, 2.2, 2.0, 1.4, 0.85, 0.6, 0.3; BamHI/HindIII--21.0, 12.0, 2.9, 2.8, 2.7, 1.6, 1.3, 1.2.
Restriction digests of the clone give the following sizes (kb): EcoRI--25.0, 11.0, 8.4, 1.0; XbaI--19.5, 11.0, 9.2, 4.0, 2.1; XhoI--uncut; SalI--20.5, 16.0, 8.5;
Detects a murine genomic EcoRI fragment of more than 23 kb.
The complete coding sequence is contained in a 2.4 kb BamHI/HindIII fragment.
Detects a human transcript of 4.8 kb.
References

Yamada Y, et al. Human somatostatin receptor genes: localization to human chromosomes 14, 17, and 22 and identification of simple tandem repeat polymorphisms. Genomics 15: 449-452, 1993. PubMed: 8449518

Yamada Y, et al. Somatostatin receptors, an expanding gene family: cloning and functional characterization of human SSTR3, a protein coupled to adenylyl cyclase. Mol. Endocrinol. 6: 2136-2142, 1992. PubMed: 1337145

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