lambdahSSTR2-17 (ATCC® 79048)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: GI Bell

Permits and Restrictions

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Designations lambdahSSTR2-17
GenBank Number

M96738

Species Homo sapiens, human
Depositors GI Bell
Vector
Construct size (kb): 44.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaFIX II
Intact vector size: 41.900
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: NotI SalI XhoI EcoRI
Polylinker sites: XbaI SacI NotI SacI SalI XhoI EcoRI XbaI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: T3, T7
replicon: lambda
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SSTR3
Gene name: somatostatin receptor 3
Contains complete coding sequence?: Y
Chromosome: 22; Localization: 22 q13.1
Tissue: placenta, male Caucasian
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 15.0
Cross references: DNA Seq. Acc.: M96738
Insert lengths(kb): 15.0
Tissue: placenta, male Caucasian
Gene product: somatostatin receptor 3 [SSTR3]
Insert Size (kb) 15.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
SacI--18.5, 8.6, 4.4, 2.5, 2.3, 2.2, 2.0, 1.4, 0.85, 0.6, 0.3; BamHI/HindIII--21.0, 12.0, 2.9, 2.8, 2.7, 1.6, 1.3, 1.2.
Restriction digests of the clone give the following sizes (kb): EcoRI--25.0, 11.0, 8.4, 1.0; XbaI--19.5, 11.0, 9.2, 4.0, 2.1; XhoI--uncut; SalI--20.5, 16.0, 8.5;
Detects a murine genomic EcoRI fragment of more than 23 kb.
The complete coding sequence is contained in a 2.4 kb BamHI/HindIII fragment.
Detects a human transcript of 4.8 kb.
References

Yamada Y, et al. Human somatostatin receptor genes: localization to human chromosomes 14, 17, and 22 and identification of simple tandem repeat polymorphisms. Genomics 15: 449-452, 1993. PubMed: 8449518

Yamada Y, et al. Somatostatin receptors, an expanding gene family: cloning and functional characterization of human SSTR3, a protein coupled to adenylyl cyclase. Mol. Endocrinol. 6: 2136-2142, 1992. PubMed: 1337145

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