182 (ATCC® 79014)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: MA Gitt

Permits and Restrictions

View Permits

Designations 182
GenBank Number

J05303

Species Homo sapiens, human
Depositors MA Gitt
Vector
Construct size (kb): 40.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJB8
Intact vector size: 5.400
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI EcoRI
Polylinker sites:
Construction: HomerI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LGALS1
Genomic copy number: unique
Gene name: lectin, galactoside-binding, soluble, 1
Contains complete coding sequence?: U
Chromosome: 22; Localization: 22 q12-q13.1
Tissue: leukocyte
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 35.0
Cross references: DNA Seq. Acc.: J05303
Insert lengths(kb): 35.0
Tissue: leukocyte
Gene product: lectin, galactoside-binding, soluble, 1 [LGALS1]
Insert Size (kb) 35.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.2, 4.2, 3.0, 3.0, 3.0, 2.35, 2.05, 1.95, 1.9, 1.7, 1.5, 1.35, 1.25, 1.1, 0.92, 0.9, 0.85, 0.85, 0.8, 0.76, 0.72, 0.48, 0.42, 0.35;
PvuII--4.6, 4.4, 4.1, 3.7, 3.7, 3.2, 2.2, 2.0, 1.9, 1.65, 1.25, 1.25, 1.0, 1.0, 0.75, 0.65, 0.6, 0.46, 0.4, 0.4; EcoRI--12.0, 8.0, 7.0, 4.5, 3.3, 1.65, 0.84; EcoRI/BamHI--8.4, 8.4, 4.5, 4.3, 3.65, 2.25, 1.85, 1.78, 1.7, 1.15, 0.8;
EcoRI/PstI--4.2, 3.3, 3.0, 3.0, 2.35, 2.2, 1.75, 1.55, 1.35, 1.2, 1.15, 1.1, 1.0, 0.95, 0.95, 0.84, 0.75, 0.68 +smaller.
The 4428 nt sequenced region includes the following restriction sites (nt from the 5' end): PstI--531, 642, 1011 (all intron 1), 1850 (intron 2); 3218 (intron 3); EcoRI--4211 (exon 4).
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The sequence record begins at a Sp1 binding site consensus approximately 260 bp 5' to the initiator ATG.
Contains the complete gene (4 exons).
Introns 2 and 3 contain Alu repeats.
References

Lau YF, Kan YW. Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin cluster. Proc. Natl. Acad. Sci. USA 80: 5225-5229, 1983. PubMed: 6310566

Gitt MA, Barondes SH. Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30: 82-89, 1991. PubMed: 1988031

Product Sheet
Product Sheet
Product Sheet