Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 6.3000001907348630
Vector: pUXV6 (plasmid)
Promoters: Promoter gap
Construction: pUXV2, S. hindustanus ble
Construct size (kb): 6.300000190734863
Features: marker(s): ampR, bleR
replicon: pMB1, UARS
Restriction digests of the clone give the following sizes (kb): EcoRI--6.25; SacI--6.35; HindIII--2.7, 3.6.
Available for research purposes only.
One of a series of vectors (ATCC 77463-77465 and 77468-77471) differing only in the selectable marker and the orientation of the gap promoter.
Differs from pUXV5 (ATCC 77468)
only in the orientation of the gap promoter and the available cloning site.
Differs from pUXV8 (ATCC 77471)
only in the source of the bleomycin resistance gene. This comes from Streptoalloteichus hindustanus.
Shuttle expression vector using Ustilago maydis promoters gap (glyceraldehyde phosphate dehydrogenase) and hsp70 to drive expression of the cloned insert and bleomycin resistance genes respectively.
The order of the major features in the plasmid is: Ustilago maydis ARS - ampR - pMB1 ori - 5' lacZ fragment - EcoRI - SacI <- gap promoter - BamHI - HindIII - hsp70 promoter -> S. hindustanus bleR -> hsp70 term - HindIII - 3' lacZ fragment.
Kinal H, et al. A family of Ustilago maydis expression vectors: new selectable markers and promoters. Gene 127: 151-152, 1993. PubMed: 8486284
Kinal H, et al. An expression vector for the phytopathogenic fungus, Ustilago maydis. Gene 98: 129-134, 1991. PubMed: 2013404