pUXV6 (ATCC® 77469)

Applications: expression vectorshuttle vector  /  Depositors: JA Bruenn

Permits and Restrictions

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Designations pUXV6
Depositors JA Bruenn
Biosafety Level 1
Vector Information
Size (kb): 6.3000001907348630
Vector: pUXV6 (plasmid)
Promoters: Promoter gap
Construction: pUXV2, S. hindustanus ble
Marker(s):ampR,bleR
Construct size (kb): 6.300000190734863
Features: marker(s): ampR, bleR
promoter: gap
replicon: pMB1, UARS
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.25; SacI--6.35; HindIII--2.7, 3.6.
Available for research purposes only.
One of a series of vectors (ATCC 77463-77465 and 77468-77471) differing only in the selectable marker and the orientation of the gap promoter.
Differs from pUXV5 (ATCC 77468) only in the orientation of the gap promoter and the available cloning site.
Differs from pUXV8 (ATCC 77471) only in the source of the bleomycin resistance gene. This comes from Streptoalloteichus hindustanus.
Shuttle expression vector using Ustilago maydis promoters gap (glyceraldehyde phosphate dehydrogenase) and hsp70 to drive expression of the cloned insert and bleomycin resistance genes respectively.
The order of the major features in the plasmid is: Ustilago maydis ARS - ampR - pMB1 ori - 5' lacZ fragment - EcoRI - SacI <- gap promoter - BamHI - HindIII - hsp70 promoter -> S. hindustanus bleR -> hsp70 term - HindIII - 3' lacZ fragment.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Kinal H, et al. A family of Ustilago maydis expression vectors: new selectable markers and promoters. Gene 127: 151-152, 1993. PubMed: 8486284

Kinal H, et al. An expression vector for the phytopathogenic fungus, Ustilago maydis. Gene 98: 129-134, 1991. PubMed: 2013404

Shipped freeze-dried
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