pTB (ATCC® 77386)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: JJ McCormick

Permits and Restrictions

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Designations pTB
Depositors JJ McCormick
Biosafety Level 1
Vector Information
Size (kb): 5.8460001945495610
Vector: pTB (plasmid)
Promoters: Promoter metallothionein
Construction: pT (ATCC 77384)
Marker(s):ampR
Construct size (kb): 5.846000194549561
Features: insert detection: lacZ'
insert detection: lacZ/amb5
marker(s): ampR
promoter: metallothionein
replicon: pMB1
MCS: EcoRI...HindIII
MCS: NruI...HindIII
restriction site: NotI
restriction site: NsiI
terminator: SV40
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PvuI--2.6, 1.8, 1.4; EcoRI--3.3, 2.5; HindIII--4.5, 1.3; BglII--5.8; ClaI--5.8.
The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 3272; SacI--1593; PvuI--1842, 3197, 4987; ClaI--1981; XhoI--2005; BglII--2028; SphI--3153, 3356; NsiI--3155; HindIII--2040, 3368; DrdI--3829, 5700; BsaI--4687.
Expression vector containing two lacZalpha-based multiple cloning sites, with the second containing an amber mutation and being flanked by a mouse metallothionein I promoter at one end and splicing and polyadenylation signals at the other.
The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed.
Both sites permit detection of inserts by alpha complementation, with discrimination between the two provided by the amber mutation in the second lacZ.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962

J J McCormick, personal communication

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