pBTGFbeta1 [bTGF-beta1 clA] (ATCC® 77321)

Organism: Bos taurus, cow  /  Clone Type: Clone  /  Depositors: S Qian

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Designations pBTGFbeta1 [bTGF-beta1 clA]
GenBank Number

M36271

Species Bos taurus, cow
Depositors S Qian
Vector
Construct size (kb): 4.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Bos taurus
Gene symbol: TGFB1
Gene name: transforming growth factor, beta 1
Contains complete coding sequence?: U
Tissue: fibropapilloma
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: M36271
Insert lengths(kb): 1.100000023841858
Tissue: fibropapilloma
Gene product: transforming growth factor, beta 1 [TGFB1]
Insert Size (kb) 1.100
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--2.9, 1.5; KpnI--4.4; BamHI--3.1, 1.4.
Insert contains the following restriction sites (approximate kb from the 5' end): BalI--0.57; KpnI--0.72; SmaI--0.82; PvuII--0.94.
A partial cDNA clone that lacks the 5' untranslated region and the first 223 nt of the precursor coding sequence but encodes the complete mature protein.
References

Van Obberghen-Schilling E, et al. Complementary deoxyribonucleic acid cloning of bovine transforming growth factor-beta1. Mol. Endocrinol. 1: 693-698, 1987. PubMed: 3153459

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