pBTGFbeta1 [bTGF-beta1 clA] (ATCC® 77321)

Organism: Bos taurus, cow  /  Clone Type: Clone  /  Depositors: S Qian

Permits and Restrictions

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Designations pBTGFbeta1 [bTGF-beta1 clA]
GenBank Number

M36271

Species Bos taurus, cow
Depositors S Qian
Vector
Construct size (kb): 4.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Bos taurus
Gene symbol: TGFB1
Gene name: transforming growth factor, beta 1
Contains complete coding sequence?: U
Tissue: fibropapilloma
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: M36271
Insert lengths(kb): 1.100000023841858
Tissue: fibropapilloma
Gene product: transforming growth factor, beta 1 [TGFB1]
Insert Size (kb) 1.100
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--2.9, 1.5; KpnI--4.4; BamHI--3.1, 1.4.
Insert contains the following restriction sites (approximate kb from the 5' end): BalI--0.57; KpnI--0.72; SmaI--0.82; PvuII--0.94.
A partial cDNA clone that lacks the 5' untranslated region and the first 223 nt of the precursor coding sequence but encodes the complete mature protein.
References

Van Obberghen-Schilling E, et al. Complementary deoxyribonucleic acid cloning of bovine transforming growth factor-beta1. Mol. Endocrinol. 1: 693-698, 1987. PubMed: 3153459

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