DQ H2.2 (ATCC® 77231)

Organism: Oryctolagus cuniculus, rabbit  /  Clone Type: Clone  /  Depositors: C Leguern, TJ Kindt

Permits and Restrictions

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Designations DQ H2.2
GenBank Number

M15557

Species Oryctolagus cuniculus, rabbit
Depositors C Leguern, TJ Kindt
Applications
The recommended probe is the 0.5 AccI/PstI fragment corresponding to nt 1166 - 1684 of the reported sequence, which begins toward the 3' end of exon 3 and extends into intron 4 (encoding through the cytoplasmic tail).
Vector
Construct size (kb): 5.0
Insert
DNA: genomic
Insert lengths(kb): 2.200000047683716
Tissue: T-lymphocyte RL-5 cell line
Gene product: major histocompatibility complex, class II, DQ alpha 1 [RLA-DQA]
Insert Size (kb) 2.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): AccI/PstI--3.2, 0.85, 0.6, 0.3; PstI--3.8, 1.25; EcoRI--3.0, 2.0.
The recommended probe is the 0.5 AccI/PstI fragment corresponding to nt 1166 - 1684 of the reported sequence, which begins toward the 3' end of exon 3 and extends into intron 4 (encoding through the cytoplasmic tail).
The complete insert begins upstream of exon 2 and continues beyond the last exon (exon 5).
Rabbit genomic PvuII fragments detected by this probe correspond to these haplotypes (kb): D1--8.0; D2--1.8; D3--5.9; D4--3; D6--6.3; D7--4.7; D8--6.3; D15--3.0; D17--8.0.
Rabbit genomic EcoRI fragments detected by this probe correspond to these haplotypes (kb): D1--2.8; D2--6.3; D3--3.7; D4--9.6; D6--2.8; D7--6.3; D8--6.3; D15--3.3; D17--3.7.
References

Marche PN, et al. Definition of rabbit class I and class II MHC gene haplotypes using molecular typing procedures. Immunogenetics 29: 273-276, 1989. PubMed: 2564845

LeGuern C, et al. Molecular evidence for five distinct MHC class II alpha genes in the rabbit. Immunogenetics 22: 141-148, 1985. PubMed: 3861576

LeGuern C, et al. Sequence determination of a transcribed rabbit class II gene with homology to HLA-DQalpha. Immunogenetics 25: 104-109, 1987. PubMed: 3493213

Thomas J Kindt, personal communication