DQ H2.2 (ATCC® 77231)

Organism: Oryctolagus cuniculus, rabbit  /  Clone Type: Clone  /  Depositors: C Leguern, TJ Kindt

Permits and Restrictions

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Designations DQ H2.2
GenBank Number

M15557

Species Oryctolagus cuniculus, rabbit
Depositors C Leguern, TJ Kindt
Applications
The recommended probe is the 0.5 AccI/PstI fragment corresponding to nt 1166 - 1684 of the reported sequence, which begins toward the 3' end of exon 3 and extends into intron 4 (encoding through the cytoplasmic tail).
Vector
Construct size (kb): 5.0
Insert
DNA: genomic
Insert lengths(kb): 2.200000047683716
Tissue: T-lymphocyte RL-5 cell line
Gene product: major histocompatibility complex, class II, DQ alpha 1 [RLA-DQA]
Insert Size (kb) 2.200
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): AccI/PstI--3.2, 0.85, 0.6, 0.3; PstI--3.8, 1.25; EcoRI--3.0, 2.0.
The recommended probe is the 0.5 AccI/PstI fragment corresponding to nt 1166 - 1684 of the reported sequence, which begins toward the 3' end of exon 3 and extends into intron 4 (encoding through the cytoplasmic tail).
The complete insert begins upstream of exon 2 and continues beyond the last exon (exon 5).
Rabbit genomic PvuII fragments detected by this probe correspond to these haplotypes (kb): D1--8.0; D2--1.8; D3--5.9; D4--3; D6--6.3; D7--4.7; D8--6.3; D15--3.0; D17--8.0.
Rabbit genomic EcoRI fragments detected by this probe correspond to these haplotypes (kb): D1--2.8; D2--6.3; D3--3.7; D4--9.6; D6--2.8; D7--6.3; D8--6.3; D15--3.3; D17--3.7.
References

Marche PN, et al. Definition of rabbit class I and class II MHC gene haplotypes using molecular typing procedures. Immunogenetics 29: 273-276, 1989. PubMed: 2564845

LeGuern C, et al. Molecular evidence for five distinct MHC class II alpha genes in the rabbit. Immunogenetics 22: 141-148, 1985. PubMed: 3861576

LeGuern C, et al. Sequence determination of a transcribed rabbit class II gene with homology to HLA-DQalpha. Immunogenetics 25: 104-109, 1987. PubMed: 3493213

Thomas J Kindt, personal communication

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