pG95/T1 (ATCC® 77079)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: A Tzagoloff

Permits and Restrictions

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Designations pG95/T1
GenBank Number

X56215

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors A Tzagoloff
Applications
in another host, produces protein mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1)
Vector
Construct size (kb): 13.80000019073486
Insert
DNA: genomic
Insert lengths(kb): 6.0
Gene product: mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1) [ATP13]
Target Gene: mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1)
Insert Size (kb) 6.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 3.7, 2.3, 1.0, 0.6; SstI--12.0, 1.7; SmaI/XhoI--9.4, 4.6; Xba--5.2, 4.8, 4.4; SalI/SmaI--7.0 (doublet).
The insert contains the following restriction sites (approximate kb from the end closer to the SalI site of the vector [nt of published sequence]): XbaI--0.5, 4.5; HindIII--1.3 [1], 2.3 [1004], 2.5 [1269]; XhoI--1.9 [656]; SstI--0.6, 2.25 [969].
EcoRI--2.5 [1227], 3.2, 4.1.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Ackerman SH, et al. ATP13, a nuclear gene of Saccharomyces cerevisiae essential for the expression of subunit 9 of the mitochondrial ATPase. FEBS Lett. 278: 234-238, 1991. PubMed: 1825065

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