pG95/T1 (ATCC® 77079)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: A Tzagoloff

Permits and Restrictions

View Permits

Designations pG95/T1
GenBank Number

X56215

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors A Tzagoloff
Applications
in another host, produces protein mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1)
Vector
Construct size (kb): 13.80000019073486
Insert
DNA: genomic
Insert lengths(kb): 6.0
Gene product: mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1) [ATP13]
Target Gene: mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1)
Insert Size (kb) 6.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 3.7, 2.3, 1.0, 0.6; SstI--12.0, 1.7; SmaI/XhoI--9.4, 4.6; Xba--5.2, 4.8, 4.4; SalI/SmaI--7.0 (doublet).
The insert contains the following restriction sites (approximate kb from the end closer to the SalI site of the vector [nt of published sequence]): XbaI--0.5, 4.5; HindIII--1.3 [1], 2.3 [1004], 2.5 [1269]; XhoI--1.9 [656]; SstI--0.6, 2.25 [969].
EcoRI--2.5 [1227], 3.2, 4.1.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Ackerman SH, et al. ATP13, a nuclear gene of Saccharomyces cerevisiae essential for the expression of subunit 9 of the mitochondrial ATPase. FEBS Lett. 278: 234-238, 1991. PubMed: 1825065

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