WFH-PLA (ATCC® 69254)

Organism: Dolichovespula maculata, white-face hornet  /  Clone Type: Clone  /  Depositors: Rockefeller Univ., TP King, Rockefeller Univ.

Permits and Restrictions

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Designations WFH-PLA
GenBank Number

X66869

Species Dolichovespula maculata, white-face hornet
Depositors Rockefeller Univ., TP King, Rockefeller Univ.
Vector
Construct size (kb): 5.043000221252441
Insert
DNA: cDNA
Insert lengths(kb): 1.14300000667572
Tissue: D. maculata venom sac acid gland
Gene product: phospholipase A1 [Dol m I]
Insert Size (kb) 1.143
Media ATCC® Medium 1634: LB medium (ATCC medium 1065) with 50 mcg/ml kanamycin
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 1.0; XbaI--5.0; HindIII--5.0.
The insert contains the following restriction sites (approximate kb from the 5' end): DraII--0.80.
Recommendation for verification: EcoRI; DraII; HindIII; XbaI.
The cDNA containing phospholipase A1 was amplified by PCR. PCR products containing single 3'-overhanging A-nucleotides were cloned into the pCR vector with compatible T-nucleotide overhangs.
References

King TP. Nucleic acid and recombinant production of vespid venom hyaluronadase. US Patent 5,593,877 dated Jan 14 1997

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