pSELECT-1 [pALTER-1] (ATCC® 68196)

Applications: vector containing primer sites useful for sequencingvector permitting RNA synthesis in vitrovector permitting visual detection of recombinantsvector useful for in-vitro mutagenesis  /  Depositors: Promega Corporation, JC Grosch, Promega Corporation

Permits and Restrictions

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Designations pSELECT-1 [pALTER-1]
Depositors Promega Corporation, JC Grosch, Promega Corporation
Biosafety Level 1
Vector Information
Size (kb): 5.6799998283386230
Vector: pSELECT-1 (phagemid)
Promoters: Promoter T7
Construction: pBR322, pGEM-3Zf(+)
Marker(s):tetR
Construct size (kb): 5.679999828338623
Features: insert detection: lacZ'
marker(s): tetR
promoter: lac, SP6, T7
replicon: pMB1, f1
Applications
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting visual detection of recombinants
vector useful for in-vitro mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.2; BamHI--6.2; HindIII--6.2.
This vector contains the following unique restriction sites: ClaI, EcoRV, StyI, ScaI and AatII.
Useful for in vitro mutagenesis experiments. Encodes the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.
Contains an inactive ampicillin resistance gene which can be reverted to functional expression by a repair oligonucleotide.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Promega Corporation, personal communication

Shipped frozen
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map