Size (kb): 11.4540004730224600
Vector: pYAC4 (YAC)
Construct size (kb): 11.45400047302246
Features: marker(s): HIS3
YL-type (YAC) shuttle vector
vector for constructing genomic libraries
Yeast artificial chromosome (YAC) vector for cloning EcoRI fragments.
A 0.7 kb BamHI-XhoI fragment contains two copies of the Tetrahymena thermophila macronuclear telomere.
This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements.
Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825
Wells RA, et al. Telomere-related sequences at interstitial sites in the human genome. Genomics 8: 699-704, 1990. PubMed: 2276741
Burke DT, Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194: 251-270, 1991. PubMed: 2005791
Kuhn RM, Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene 141: 125-127, 1994. PubMed: 8163163