pYAC4 (ATCC® 67379)

Applications: YL-type (YAC) shuttle vectorShuttle vectorVector for constructing genomic libraries  /  Depositors: MV Olson

Permits and Restrictions

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Designations pYAC4
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors MV Olson
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 11.4540004730224600
Vector: pYAC4 (YAC)
Marker(s): TRP1,URA3,ampR,HIS3,SUP4
Construct size (kb): 11.454
Features: marker(s): HIS3
marker(s): SUP4
marker(s): TRP1
marker(s): URA3
marker(s): ampR
other: telomere
replicon: ARS1
replicon: pMB1
centromere: CEN4
YL-type (YAC) shuttle vector
Shuttle vector
Vector for constructing genomic libraries
Yeast artificial chromosome (YAC) vector for cloning EcoRI fragments
A 0.7 kb BamHI-XhoI fragment contains two copies of the Tetrahymena thermophila macronuclear telomere
This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825

Wells RA, et al. Telomere-related sequences at interstitial sites in the human genome. Genomics 8: 699-704, 1990. PubMed: 2276741

Burke DT, Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194: 251-270, 1991. PubMed: 2005791

Kuhn RM, Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene 141: 125-127, 1994. PubMed: 8163163

Shipped frozen
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